Ambrozine Trent-Johnney was born in St. Vincent, Grenadines. As a teenager she and her family moved to New York City, NY. Ambrozine graduated as valedictorian of her class from Prospect Height High School. She completed the physician assistant program and received her Bachelor of Science degree in 2000 from the Harlem Hospital Sophie Davis Biomedical Education, CUNY. In 2005 Ambrozine, her husband Jeffrey and son relocated to Georgia where she joined the practice of Harper and Associates Family Medicine, P.C. in early 2007.
Mrs. Trent-Johnney currently has a membership in the American Academy of Physician Assistants and also a lifetime member of Golden Key National Honors Society.